Indian Society of Genetics & Plant Breeding

Assessment of genetic diversity in cultivated groundnut (Arachis hypogaea L.) with differential responses to rust and late leaf spot using ISSR markers

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Inter simple sequence repeat (ISSR) markers were used
to analyze genetic diversity among twenty cultivated
groundnut (Arachis hypogaea L.) genotypes. Out of 154
amplicons produced by 21 ISSR primers, 115 were found
polymorphic (74.67%). The 3’-anchored primers based on
poly ‘GA’ and poly ‘AG’ motifs produced higher proportion
of polymorphism of 74.85% and 77.27%, respectively. The
primer UBC 810 identified 18 genotypes out of 20 studied
as it had the highest resolving power among the 21
primers. The dendrogram obtained from Ward method of
cluster analysis revealed four subclusters, of which
subcluster A and subcluster D contained resistant and
susceptible genotypes, respectively. Based on KruskalWallis one-way ANOVA, UBC 810540 was found associated
with both rust and late leaf spot (LLS) resistance and UBC
810500 with LLS resistance.

Keywords: Groundnut, foliar diseases, ISSR markers, PIC, resolving power


Year: 2009
Volume: 69
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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