Indian Society of Genetics & Plant Breeding

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Assessment of genetic diversity in aromatic rices (Oryza sativa L.) using morphological, physicochemical and SSR markers

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Genetic diversity of a set of 50 elite rice genotypes was assessed through morphological, quality and SSR markers. The mean values for agro-morphological and quality characters were highly significant. Plant height was significantly associated with days to 50 per cent flowering and maturity. Kernel length was highly significant positive correlation with cooked kernel length (CKL), cooked kernel breadth (CKB), gelatinization temperature (GT) and length to breadth (L/B) ratio while GT had significant negative correlation with alkali spread value (ASV) and positive correlation with CKL and L/B. Cluster analysis using morphological and physiochemical traits revealed five and three major clusters, respectively. Euclidian distance ranged from 0.39 to 24.30 per cent and 0.4 to 5.44 per cent, respectively for morphological and quality traits. SSR marker analysis revealed distinct polymorphism among the cultivars.The polymorphic information content (PIC) value of the 28 primers ranged from 0.139-0.99 with an average of 0.589 per primer. UPGMA Cluster analysis grouped the rice genotypes into eight clusters in which genetically similar type clustered together. Principal coordinate analysis revealed similar results. The study revealed that cluster analysis for related cultivars as revealed by morphological, physiochemical and microsatellites markers provides greater confidence for the assessment of genetic diversity and relationships, which could be used in subsequent breeding programs.
 

Keywords: Aromatic rice, genetic diversity, micro-satellite markers

Info

Year: 2011
Volume: 71
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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S. P. Singh info_circle
J. Kumar info_circle

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