Indian Society of Genetics & Plant Breeding

Assessment of genetic diversity based on agro-morphological traits and genic microsatellite markers in inter-specific derivatives and cultivars of pigeonpea

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In the present study, genetic diversity among 73 genotypes
comprising of cultivars and stable inter-specific derivatives
from pigeonpea (Cajanus cajan x C. scarabaeoides) was
assessed based on thirteen agro-morphological and quality
traits and 42 genic microsatellite markers. Cluster analysis
based on Tocher’s methods of pooled fielddata of agromorphological
traits from all three environment and two
locations grouped pigeonpea genotypes into nine clusters.
Genetic divergence was mainly contributed by days to 50%
flowering (30.14%) followed by100 seed weight (20.78%)
and protein content (12.56%). The range of polymorphic
information content (PIC) for microsatellites was 0.055-
0.695. A total of 104 alleles were amplified with 42 SSR
markers with an average of 2.48 alleles per locus.Ten alleles
were unique to inter-specific derivatives. Based on
Shannon’s information index (I), ASSR 17 was the most
informative locus for all the genotypes. Two loci, ASSR114
and ASSR317 were polymorphic only among the interspecific
derivatives. Cophenetic correlations were
statistically significant for phenograms based on Euclidean
distances and average Manhattan distance analysis.The
first three principal coordinates explained 60.17% and
57.22% of the accumulated variation in case of agromorphological
traits and microsatellite data respectively.
The information accrued is potential for broadening the
genetic base in pigeonpea.

Keywords: Average Manhattan distances, Cajanus scarabaeoides (L.) Millspaugh, genic microsatellites, inter-specific derivatives


Year: 2015
Volume: 75
Issue: 2
Article DOI: 10.5958/0975-6906.2015.00033.4
Print ISSN: 0019-5200
Online ISSN: 0975-6906


R. S. Raje, Anand K. Singh, A. M. Singh info_circle
Avinash Pandey info_circle
N. K. Singh info_circle

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