Indian Society of Genetics & Plant Breeding

Anther culture of Glycine max (Merr.): Effect of media on callus induction and organogenesis

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The present study was undertaken to standardize the
doubled haploid regeneration protocol for soybean. Studies
on association between floral bud size and pollen
developmental stage revealed that the bud size of 2.5-4
mm containing microspore at uni-nucleate stage was most
responsive for callus induction and organogenesis. Effect
of different media on callus induction and plant
regeneration in anther culture of three genotypes, namely,
JS 335, SL 958 and SL 688 were studied. Combination of
10.0 mg l–12, 4-D and 0.5 mg l–1 BAP supplemented with 90
g l–1 sucrose and 10% v/v of coconut water in B5 medium
was found best for callus induction (64.11%). Murashige
and Skoog medium supplemented with 2.0 mg l–1 BAP + 1.0
mg l–1 thidiazuron gave shoot bud-like structures, in
contrast, B5 medium promoted only root organogenesis
(3.30-10.90%). Anther derived callus was confirmed through
flow cytometry and root tip cell of anther callus derived
roots to be haploid through root tip staining (chromosome
number n = 20).

Keywords: Anther culture, regeneration, Glycine max (L.) Merrilly, microspore, haploid


Year: 2016
Volume: 76
Issue: 3
Article DOI: 10.5958/0975-6906.2016.00048.1
Print ISSN: 0019-5200
Online ISSN: 0975-6906


S. K. Lal info_circle
S. K. Singh info_circle
A. Talukdar info_circle
Vinod info_circle

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