Indian Society of Genetics & Plant Breeding

Analysis of genetic diversity among the Indian bread wheat cultivars using microsatellite (SSR) markers

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Genetic diversity among 35 wheat cultivars was evaluated
using 30 polymorphic microsatellite (SSR) markers. Analysis
of molecular diversity revealed moderate levels of
polymorphism in the set of genotypes studied. Ninety
alleles with an average of three alleles per locus were
detected. The allelic polymorphism information content
(PIC) value ranged from 0.06 to 0.76 with an average of 0.45.
The primers like Xgwm294, Xgwm146, Xgwm455, Xgwm497,
Xgwm136 and Xgwm132 could be considered particularly
informative, as they revealed four or more alleles per locus
and displayed high PIC values.Ten rare and three unique
alleles were recorded. The most closely related cultivars
were ‘Raj3765’ and ‘HD 2402’ displaying the lowest
dissimilarity index, whereas highest genetic distance was
observed between ‘Pissi Local’and‘HB 208’.The land race
‘Pissi Local’ revealed very high genetic distance with most
of the cultivars where the dissimilarity indices ranged from
0.42-0.74. Three major clusters comprising 13, 12 and 10
genotypes were formed. Despite the presence of variability
among the different Indian cultivars the varieties developed
at various institutes during different time periods clustered
together indicating the use of common genotypes in the

Keywords: SSR, microsatellites, molecular markers, wheat


Year: 2014
Volume: 74
Issue: 4
Article DOI: 10.5958/0975-6906.2014.00877.3
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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