Indian Society of Genetics & Plant Breeding

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Analysis of chalcone synthase gene in chemically induced male sterility (CIMS) in wheat (Triticum aestivum L.)

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Chemically induced male sterility (CIMS) is one of the male
sterility system used for hybrid wheat production in China.
However, the mechanism of sterility remains unclear. The
expression of chalcone synthase (CHS) gene was assessed
in anthers to determine its role in fertility/sterility in wheat.
The expression levels of CHS gene was evaluated by realtime
quantitative polymerase chain reaction (qPCR). qPCR
analysis revealed that the expression levels of CHS gene
were downregulated in CIMS. The sporopollenin contents
in anthers underwent a sharp decrease in 1376-CIMS.
Scanning electron microscopy observations revealed that
pollen wall formation was significantly altered in 1376-CIMS.
This phenomenon implied that CHS gene expression was
inhibited during the fertility transformation process and
was related to the transformation from fertility to sterility.
Moreover, results indicated that CHS plays an essential
role in conducting fertility in chemically interfered male
sterility.

Keywords: Male sterility, chalcone synthase gene, pollen wall, sporopollenin, wheat

Info

Year: 2017
Volume: 77
Issue: 4
Article DOI: 10.5958/0975-6906.2017.00067.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Gaisheng Zhang info_circle
Guiping Li info_circle
Lijuan Zhou info_circle
Gensheng Zhang info_circle
Zhaolin Fu info_circle
Chu Chen info_circle
Yunxian Song info_circle

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