Indian Society of Genetics & Plant Breeding

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An improved in vitro germination medium for recalcitrant bread wheat (Triticum aestivum L.) pollen

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There is no robust and quick technique available for testing
germination of wheat pollen. In this study, Brewbaker and
Kwack (BK) pollen germination medium (PGM) was
adjusted with a-amino caproic acid (EACA), peptone water
and maltose to develop an improved medium for wheat.
Germination of pollen in this PGM ranged from 92% to
98.2% depending upon genotype pollen germination media
(PGM). This is the first report of PGM which has >95% wheat
pollen germination in vitro.

Keywords: Recalcitrant wheat pollen, peptone water, maltose, e-amino caproic acid (EACA), in vitro pollen germination

Info

Year: 2015
Volume: 75
Issue: 4
Article DOI: 10.5958/0975-6906.2015.00072.3
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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J. Kumar info_circle
K. Saravannan info_circle

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