Indian Society of Genetics & Plant Breeding

An improved cytogenetic technique intended for macroalgal chromosomes

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The selection of fixative was exercisedTherefore, Different
fixatives such as acetic acid, acetone, chloroform,
methanol, ethanol and formaldehyde were used separately
or in combination in altered ratios to optimize it for
chromosome staining in macroalgae (seaweeds).
Combination of acetic acid with formaldehyde (1:1) was
found to be effective for chromosomes staining in
Chlorophyta and Rhodophyta. Moreover, the corbol
fuchsins and aceto-iron–haematoxylin-chloral-hydrate
were found to be effective stains for Rhodophyta and
Chlorophyta respectively. These techniques were used to
prepare karyological studies in seaweeds including seven
Ulva spp., Gracilaria spp., Monostroma spp. and a few
Porphyra species.

Keywords: Chromosome, chlorophyta, rhodophyta, cytogenetic, macroalgae


Year: 2012
Volume: 72
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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