In durum wheat genotypes, analysis of seed storage
protein profiles of 30 genetic stocks and 30 advanced
lines was done using Acid and 50S PAGE electrophoresis.
More alleles were observed at GJu-1 loci i.e., GJu A 1 (2*)
and GJu 81 (14+15, 7*+8 and a new type allele) in genetic
stocks, while these alleles were not noticed in advanced
lines. Most of the advance lines show null and 7+8
bands, while in genetic stocks, band 20 is common.
Genetic stocks contain 12 different patterns at GJu-3 loci,
while only two were found in advance lines, indicating
that the genetic stocks contain more diversity for this
class of proteins in comparison to advance lines. The
rust resistant lines do not posses LMW 2 I y-45 type GJu
3 I GJi-1 alleles, so these can be used as donors to
introduce rust resistance in the good quality recently
released varieties. Most of the advance lines showed
LMW 2 I y-45 type GJu 3 I GJi-1 alleles, which is best for
pasta making quality. So, LMW 2 I y-45 type GJu 3 I
GIi-1 aliele can be used as a bio-chemical marker to
select good quality lines in future breeding programmes.
The presence of new LMW-B glutenin and y-gliadin patterns
is interesting and needs to be investigated further for
their role in pasta making as well as overall technological
quality of durum wheat.
Keywords: Triticum durum, gliadin and glutenins
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
S. V. Sai Prasad, V. S. Rao and H. N. Pandey info_circle