Indian Society of Genetics & Plant Breeding
Determination of maintainer and restorer status of
germplasm collections is a prerequisite for their use in
development of hybrid employing CMS system. In the
present study, restorer and maintainer status of 372 Indian
genotypes was determined using fertility restoration (Rf)
linked microsatellite marker RM6100. A total of seven alleles
for the marker locus, including four novel and three known,
were identified in the study material. Maximum frequency
was observed for the 144bp allele (44.89%) while minimum
frequency was for 138bp allele (0.27%).Though most of the
novel alleles were minor, occurring in less than 5%
frequency, cumulatively they accounted for 9.2% frequency
in the study material. Of the known alleles, which were
found in 90.8% (338 genotypes) of the tested rice genotypes,
36% had maintainer type allele (132bp allele similar to Pusa
6B) while the rest had restorer type (144 or 150 bp) alleles.
The spikelet fertility data generated on a subset of the study
material agreed well with the established associations for
the already reported alleles. Of the novel alleles, two (141
and 147 bp) were represented in the spikelet fertility dataset,
both of which restored fertility, though their representation
was low. This allelic information would be useful in
selection of parental lines for development of hybrids in
rice.
Keywords: Cytoplasmic male sterility, hybrid, microsatellite marker alleles, rice, RM6100
Year: 2014
Volume: 74
Issue: 4
Article DOI: 10.5958/0975-6906.2014.00863.3
Print ISSN: 0019-5200
Online ISSN: 0975-6906
Kapil K. Tiwari info_circle
Sasmita Pattnaik, Ashutosh Singh info_circle
Maninder Sandhu, S. V. Amitha Mithra, M. Z. Abdin info_circle
Ashok K. Singh info_circle
Trilochan Mohapatra info_circle
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