Indian Society of Genetics & Plant Breeding

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A simple approach based on intron length polymorphism (ILP) for chromosomal localization of Gene WAG-2

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Intron polymorphism can be used to develop potential
intron polymorphism (PIP) markers. Intron length
polymorphism (ILP) is one of the most easily identified PIP
markers. WAG-2, a class C MADS-box gene, had been
isolated from the wheat expressed sequence tag (EST)
database. Rich polymorphisms, such as intron allele
variation, and length polymorphism occur in WAG-2 gene
introns. Especially, a significant length polymorphism is
shared by intron IV. In present study, based on intron IV
length polymorphism, using wheat nullisomic-tetrasomic
(NT) and ditelosomic (DT) lines, a pair of ILP primers were
designed in exons flanking introns IV and the WAG-2 gene
was located on the short arm of homologous group 3
chromosome in the wheat genome. The gene location
approach by using ILP markers combining with aneuploidy
is convenient, reliable, specific, codominant, and stable. It
is expected that ILP markers will play important roles in the
genetic studies and breeding of wheat.

Keywords: WAG-2, intron length polymorphism, chromosomal localization, nullisomictetrasomic, ditelosomic

Info

Year: 2015
Volume: 75
Issue: 3
Article DOI: 10.5958/0975-6906.2015.00049.8
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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