Indian Society of Genetics & Plant Breeding

A comparative study of Inter Simple Sequence Repeat (ISSR), Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) loci in assessing genetic diversity in Amaranthus

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Genetic diversity in a set of 31 Amaranthus accessions was
analysed using RAPD, ISSR and SSR markers. Polymorphic
Information Content (PIC) values of the polymorphic RAPD,
SSR and ISSR markers ranged from 0.067 to 0.471, 0.178 to
0.452 and 0.11 to 0.362 with an average of 0.229, 0.371 and
0.441 per primer, respectively. Gene diversity for RAPD, SSR
and ISSR markers respectively ranged from 0.032 to 0.487,
0.11 to 0.362 and 0.104 to 0.384. The similarity metrics values
for the accessions ranged from 0.58 to 0.98 with RAPD, 0.70
to 1.00 with SSR and from 0.44 to 0.88 with ISSR markers.
The dendrogram generated by the markers had five major
clusters in case of RAPD markers, two distinct clusters in
case of SSR markers and six clusters in case of ISSR markers.
Amongst all, ISSR markers provided greater confidence for
the assessment of genetic diversity in comparison to SSR
and RAPD markers. All the accessions studied were found
genetically diverse which may be useful in breeding for
improving the genotypes of Amaranthus.

Keywords: Amaranthus, genetic diversity, molecular markers, principal coordinate analysis

Info

Year: 2013
Volume: 73
Issue: 4
Article DOI: 10.5958/j.0975-6906.73.4.062
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Shailesh Pandey info_circle
J. Kumar info_circle

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