Indian Society of Genetics & Plant Breeding

Important

USE OF MOLECULAR MARKERS FOR DIVERSITY ANALYSIS IN RICE

Published on

The genetic relationship between seven japonica, two indica and one tropical japonica
rice varieties was analysed by using PCR with Random Amplified Polymorphic DNA
(RAPD) and Amplified Fragment Length Polymorphism (AFLP)' methods. In RAPD
analysis PCR with 10 arbitrary primers applied to ten rice varieties produced 84 useful
markers, of which 77.4% were polymorphic. Fifteen AFLP primer combinations produced
285 markers, of which 70.8% were polymorphic. Thus, sufficient polymorphism could
be detected to allow identification of individual varieties. Visual examination of
electrophoresis gels and· analysis of banding patterns confirmed that all the seven
japonica types were closely related, with similarity indices of 5()..85%. Two indica varieties
were classified into separate group. However, the tropical japonica type was easily
distinguished, producing variety specific amplification profiles and expressing a lower
similarity index to all other varieties tested. Thus, both RAPD and AFLP methods
offer a potentially simple, rapid and reliable method for rice genotype identification
and recognition of lines that could contribute genetic diversity to new commercial
varieties. AFLP was more useful than RAPD because the potential number of loci that
could be assayed with AFLP far exceeds that with RAPD.
 

Keywords: Rice, Oryza sativa, RAPD, AFLP, genetic diversity

Info

Year: 1999
Volume: 59
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle

Download PDF