Indian Society of Genetics & Plant Breeding
The role of growth regulators and glutamine amino acid
on regenerative ability from young and old callus cultures
of high yielding cultivars of wheat was studied. Callus
was induced from immature embryo explants of 1-1.5 mm
diameter on Murashige and Skoog (MS) basal medium
supplemented with 2 mgll 2, 4-0. Shoot formation was
obtained from young calli (10-12 week old) on MS medium
without growth regulators. From young calli increase in
regeneration frequency was obtained when cytokinin
concentration was increased from 1 to 2 mgn. The addition
of auxin to cytokinin containing media further increased
the regeneration frequency. When the medium contained
2mg/l cytokinin and 0.5 mg/l NAA, an average regeneration
frequency of 94% was obtained. Regeneration from old
calli (28-30 week old) was obtained only when the medium
contained growth regulators. There was a progressive
increase in regeneration frequency with the increase in
cytokinin concentration from 1 to 3 mgll. The same
cytokinin medium when suplemented with low levels of
auxin showed 3·6% increase in regeneration frequency.
There was pronounced effect of glutamine on old callus
cultures in increasing the regenerative ability by 6 to 10%
when cytokinin or cytokinin- auxin containing media were
supplemented with glutamine. Shoot induction frequency
of 55% could be obtained from old callus cultures in a
medium containing 3 mgll cytokinin, 0.5 mgll auxin and
150 mg/l glutamine.
Keywords: Wheat, callus, glutamine, growth regulators, regeneration
Year: 2001
Volume: 61
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
S. Shrivastava and H. S. Chawla info_circle
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