Indian Society of Genetics & Plant Breeding

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STUDY ON EFFECT OF GENOTYPE AND CULTURE MEDIUM ON CALLUS FORMATION AND PLANT REGENERATION IN RICE (ORYZA SATIVA L.)

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A study on the effect of genotype and culture medium on callus induced in seed explants
of ten rice cultivars indicated significant variances due to genotypes, media and genotype
x medium interactions (except callus induction frequency) for eight characters related to
callusing and plant regeneration. Among the genotypes, Pusa Basmati 1 gave the best
overall callusing response. For plantlet regeneration, variety Pant Dhan 4 recorded the
highest number of green spots and shoots while Sarju 52 had the highest potential for the
length of longest shoot and rooting. Likewise the MS medium supplemented with
2.0 mgl- t, 2,4-0 for callusing and 2.0 mgl- t IAA plus 3.0 mgl- t KN (MS (3)) for shooting
while 3.0 mgl- t IAA plus 4.0 mgr 1 KN (MS (4)) for rooting gave the best results. Some of
the selected combinations like Pant Ohan 4 on MS medium supplemented with 2.0 mgl- t
2,4-0 plus 1.0 mgr t KN displayed excellent callusing capacity while Pant Ohan 4 on MS
(3) and MS (4), Sarju 52 on MS (4) and Pusa Basmati 14.0 mgr t lAA plus 4.0 mgl- t KN (MS
(5)) exhibited best regeneration capacity, and therefore, offer good scope for high
totipotency using appropriate genotype and media with proper combination of hormones.
 

Keywords: Oryza sativa L., callus growth, plant regeneration, tissue culture.

Info

Year: 1994
Volume: 54
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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