Forty five Genbank derived STMS primers were tested on
36 sugarcane (Saccharum spp.) varieties and genetic
stocks to Identify the most suitable primers for clonal
identification in sugarcane. Initially, molecular profiles of
25 elite hybrids of diverse origin were generated with
these primers to identify those with high polymorphism
information content. Each hybrid could be distinguished
individually by twenty primers, while the remaining primers
were effective in combination of two or three. The extent
of genetic differences brought about by somaclonal
variation and Induced mutation in sugarcane was
significantly less compared with that brought about by
conventional breeding. Based on PIC values, 25 most
polymorphic STMS primers were Identified for testing on
genetically more similar genotypes Including six
somaclones and five Induced mutants derived from a
sugar-rich variety viz., CoC 671. Highly similar molecular
profiles of these clones reflected their similar genetic
architecture. Seventeen primers detected genetic
differences among the mutants, In contrast to only three,
among the somaclones Indicating that Induced mutations
created more genetic changes than somaclonal variations.
Thirteen most suitable primers that could differentiate
commercial hybrids as well as the mutants and somaclones
were Identified for routine fingerprinting of sugarcane
hybrids
Keywords: Sugarcane, STMS primers, molecular fingerprinting, hybrids, somaclones, induced mutants
Year: 2006
Volume: 66
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
G. Hemaprabha, P. Govindaraj and N. K. Singh info_circle