Indian Society of Genetics & Plant Breeding
Somatic embryogenesis was obtained from long term callus cultures of leaf bud in
half strength MS medium supplemented with both 0.5 mgt1 2,4-0 +1.0 mgll NAA
and 0.5 mgll 2,4-0. However, further development of the somatic embryos into
plants was obtained only from the 0.5 mgll 2,4-0 supplemented cultures. Half
strength MS medium supplemented with 0.5 mgll 2,4-0 + 1.0 mgll NAA + 0.5 mgll
BAP yielded only non-embryogenic callus. Isozyme patterns were studied to
distinguish between non-embryogenic and embryogenic calli and also between
non-viable and viable somatic embryos in embryogenic calli. Specific isoperoxidases
and isoesterases were found to be associated with the development of viable somatic
embryos.
Keywords: Somatic embryogenesis, isozyme analysis, rose, micropropagation
Year: 1998
Volume: 58
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
N. JAYASREE B. PRATIllBHA DEVI AND N. VIJAYA info_circle
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