Indian Society of Genetics & Plant Breeding

Important

SOMATIC EMBRYOGENESIS AND ISOZYMES IN ROSE SPECIES ROSA HYBRIDA L. CV. KING'S RANSOM

Published on

Somatic embryogenesis was obtained from long term callus cultures of leaf bud in
half strength MS medium supplemented with both 0.5 mgt1 2,4-0 +1.0 mgll NAA
and 0.5 mgll 2,4-0. However, further development of the somatic embryos into
plants was obtained only from the 0.5 mgll 2,4-0 supplemented cultures. Half
strength MS medium supplemented with 0.5 mgll 2,4-0 + 1.0 mgll NAA + 0.5 mgll
BAP yielded only non-embryogenic callus. Isozyme patterns were studied to
distinguish between non-embryogenic and embryogenic calli and also between
non-viable and viable somatic embryos in embryogenic calli. Specific isoperoxidases
and isoesterases were found to be associated with the development of viable somatic
embryos.
 

Keywords: Somatic embryogenesis, isozyme analysis, rose, micropropagation

Info

Year: 1998
Volume: 58
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle

Download PDF