Chlorophyll content in leaf which can be measured and
expressed as SPAD values, is a good indicator of plant
health. The present study aimed to develop linear models
for studying relationship between SPAD values and
spectrophotometrically determined chlorophylls and
carotenoid contents in leaves of 468 wheat genotypes. The
SPAD values were taken in the flag leaves at heading stage.
Chlorophyll a (Chl a), chlorophyll b (Chl b), total chlorophyll
(total Chl) and total carotenoids (total Car) contents were
assessed using dimethyl sulfoxide (DMSO) extraction
method. To estimate photosynthetic pigment content in
leaves, SPAD value-based linear models were developed
and Chla (Chlb = 0.0690 x SPAD Value - 1.082), Chla (Chlb =
0.021 x SPAD Value 0.396), total Chl (Total chlorophyll =
0.090 x SPAD Value - 1.477) and total carotenoids (Total
Carotenoids = 0.013 x SPAD Value 0.074) were estimated.
The present study reports development of linear model for
quantification of the photosynthetic pigment content in
wheat leaves using chlorophyll meter.
Keywords: Carotenoids, chlorophyll, photosynthetic pigments, SPAD measurement, wheat
Article DOI: https://doi.org/10.31742/IJGPB.79.1.13
Print ISSN: 0019-5200
Online ISSN: 0975-6906
Pramod Kumar and R. K. Sharma info_circle