Indian Society of Genetics & Plant Breeding

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Seed protein characterization and isozyme diversity for cultivar identification in grasspea (Lathyrus sativus L.)

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The characterization of seed protein of various
geographically divergent grasspea genotypes was done
by protein fractionation, polymorphism and isozymic
analysis. No positive correlation was found between seed
size and protein content. Though there was genotypespecificity for different levels of protein fractions, globulin
level proved to be the highest and prolamine the least.
50S-PAGE of different grasspea genotypes, even of same
geographic origin revealed variation in number, width and
intensity of bands. The multigene inheritance of seed
protein expression could be identified from the zymograms.
Similarity percentage between genotypes was also
calculated. In general, geographical origin does not
influence genotype specific seed protein content and its
polymorphism. Observations suggested the polymorphic
nature of esterase and peroxidase isozyme, which provided
worthy information, regarding genetic variability among
the cultivars under experiment.
 

Keywords: Grasspea, seed storage protein, protein fraction, protein polymorphism, isozymes.

Info

Year: 2001
Volume: 61
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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