Indian Society of Genetics & Plant Breeding


Root acidification, a rapid method of screening soybean genotypes for low-phosphorus stress

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Carboxylate efflux in response to low-phosphorus (P) is
one of the plant’s adaptive strategies. Genotypic variation
between the type and amount of root-exuded carboxylates
determines the differential P acquisition efficiency (PAE) in
soybean (Glycine max (L.) Merrill.). We compared direct
(using HPLC) and indirect methods (total carbon exudation,
and use of bromocresol purple dye) for quantifying root
acidification. Total carbon exudation did not correlate to
carboxylate efflux, whereas a significant linear relationship
between exudate pH and carboxylate concentration
suggested that measure of root acidification might predict
the genotypic potential for low-P induced carboxylate

Keywords: Acidification, bromocresol purple, carboxylate efflux, phosphorus, root exudate


Year: 2016
Volume: 76
Issue: 2
Article DOI: 10.5958/0975-6906.2016.00025.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906


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