Indian Society of Genetics & Plant Breeding

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RFLP FINGERPRINTING OF ORYZA AUSTRALIENSIS GENOTYPES USING CULTIVATED RICE GENOMIC PROBES

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Four accessions of O. australiensis with EE genome were examined for restriction fragment
length polymorphism (RFLPs). Fifteen RFLP clones of O. sativa, with known chromosomal
positions showed RFLPs among the accessions. However, 54.60/. monomorphic banding
patterns indicated conserved nature of DNA of O. australiensis genome. The differences
in electrophoretic banding patterns were parsimoniously interpreted as the result of 108
mutations. Three RFLP clones (RGll8, RG190, RG207) revealed a number of RFLPs among
the accessions which could fingerprint the four genotypes. The genetic relationship
between the accessions were determined by the positions of the accessions in the
phylogenetic tree and by computation of mutation distances between the accessions. We
found that the accession 100882 is closer to the accession 101397 in terms ofgenetic distance
than the accessions 103318 and 101410.
 

Keywords: RFLP, Oryza, O. australiensis, DNA fingerprint, phylogeny, genotype.

Info

Year: 1993
Volume: 53
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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