Indian Society of Genetics & Plant Breeding

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RAPD-based assessment of genetic diversity in cotton (Gossypium hirsutum L.) race stock accessions

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Twenty-five cotton (Gossypium hirsutum) accessions from
Africa, Australia, USA and India were sUbjected to RAPD
analysis using 86 random oligonuceotide primers. The
major objectives of the study were to study the extent
of genetic variation and find out the duplicates if any.
Sixty three primers detected polymorphism. A total ot 296
DNA fragments were generated by the 63 primers, of
which 113 were polymorphic. The accessions revealed
genetic divergence ranging from 0.13 to 0.33 among
themselves. RAPD analysis using SIMQUAL·Dice
Coefficient of NTSYS-pc showed that the 25 accessions
could be split into 2 groups of 24 and 1 accessions at
67% similarity. The first group of 24 accessions could be
divided into cluster A consisting of 20 accessions and
cluster 8 consisting of 4 accessions. Accessions AC 53
and AR 43 were found to be 100 percent similar based
on molecular analysis, but could not be considered as
duplicate, because of very low reproducibility of the RAPD
markers.
 

Keywords: Cotton, RAPO, genetic diversity

Info

Year: 2004
Volume: 64
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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