Indian Society of Genetics & Plant Breeding


Quantitative trait loci for mature embryo culturability traits from Yuanjiang common wild rice (Oryza rufipogon Griff.)

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Low callus subculture and regeneration ability are the main
factors restricting the genetic transformation potential of
indica rice varieties. In the present study, an Oryza
rufipogon introgression line population in the background
of indica cultivar 93-11 was used to evaluate mature embryo
culturability after callus induction, two-round of subculture,
and differentiation of culture. A total of 25 quantitative trait
loci related to tissue culturability were detected that included
QTLs for callus browning index after one- and two-round
subculture and callus regeneration rate. Among them, QTLs
detected at the RM335 locus on chromosome 4 were found
to control callus browning index after one- and two-round
subculture and accounted for 11 and 15 % of the phenotypic
variation. QTLs detected at the RM341 locus on chromosome
2 and at the RM8206 on chromosome 9 were found to control
regeneration rate with 10 % contributions to the observed
variation, respectively. The alleles of O. rufipogon were
found to reduce callus browning and were possibly involved
in the improvement of callus differentiation ability. Six
introgression lines with excellent culturability
characteristics were identified. It is expected that these
lines will be useful materials for genetic transformation
and fine mapping of culturability-trait.

Keywords: Common wild rice, callus browning, callus regeneration, QTL, genomic region


Year: 2016
Volume: 76
Issue: 2
Article DOI: 10.5958/0975-6906.2016.00037.7
Print ISSN: 0019-5200
Online ISSN: 0975-6906


Zheng Yin info_circle
Xu Xu info_circle
Chao Pu info_circle
Qi Li info_circle
Di Wu info_circle
Chuanqing Sun info_circle
Yongcai Fu info_circle

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