Indian Society of Genetics & Plant Breeding

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Molecular tagging of Rf genes for the fertility restoration of WA-CMS system by bulk segregant analysis in rice

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The process of screening for fertility restoration trait
involves test crossing with a set of cytoplasmic male sterile
(CMS) lines and evaluation of F
1 hybrids for pollen and
spikelet fertility. In the present study, F
2 mapping population
derived from a cross, APMS 6A × RP 5933-123 was utilized
to map
Rf genes. The F2 population was also genetically
analysed for pollen and spikelet fertility percentage. Chisquare (
χ2) analysis to showed that the fertility restoration
trait followed expected digenic ratio. By bulk segregant
analysis (BSA) likely
Rf genes containing regions were
located on chromosome 10. The SSR markers
viz., RM304,
RM258 located on chromosome 10 and RM23958 located
on chromosome 9 showed clear polymorphism between
two groups of fertile and sterile bulks. Based on BSA linkage
analysis and F
2 population, pollen and spikelet fertility
analysis along with molecular screening results of
Rf linked
markers, it is concluded that
Rf4 gene located on
chromosome 10 is playing major role and contributing to
90% of fertility restoration trait of newly derived restorer
line RP5933 along with minor effect genes from chromosome
9. The findings may be useful for rice hybrid breeding.

Keywords: Hybrid rice, Rf genes, WA-CMS system, BSA, Fertility restoration

Info

Year: 2021
Volume: 81
Issue: 1
Article DOI: 10.31742/IJGPB.81.1.4
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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