Molecular characterization of Indian Dolichos bean (Lablab purpureus L. var. typicus Prain) accessions using RAPD markers

Published on Jun 28, 2021 11:57 AM

In this study, 29 genotypes of (Lablab purpureus) Indian
bean augmented from different geographical regions were
analyzed for genetic diversity using 39 RAPDs. The binary
matrix data revealed 97.4% polymorphism among
genotypes, with 2322 amplification fragments. The PIC
values ranged from 0.25 to 0.96 with an average of 0.70.
Markers OPA05, OPB15, and OPV12 showed high
discrimination among all the genotypes. A magnificent
range of Jaccard’s genetic similarity coefficient was
evaluated and UPGMA based dendrogram classified
genotypes into 4 major clusters. IC-384066 and NSJ/NAIP/
192 were found to be unique and highly diversified. The
same grouping patterns were also observed in the modelbased STRUCTURE analysis (K=2). The study showed the
degree and distribution of genetic diversity in Dolichos
bean, which can be utilized to identify the parental lines, to
develop mapping populations and breeding.

Keywords: Dolichos bean, genetic diversity, RAPD, STRUCTURE, UPGMA,

Info

Year: 2021
Volume: 81
Issue: 2
Article DOI: 10.31742/IJGPB.81.2.15
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

Saidaiah Pidigam, Suchandranathbabu Munnam, Srinivas Nimmarajula, Geetha Amarapalli, Harikishan Sudini, Someshwar Rao Pandravada and Hari Yadla

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