Indian Society of Genetics & Plant Breeding

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Molecular characterization and phylogenetic assessment of a few Dioscorea (Dioscoreaceae) species of North-East India

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Dioscorea spp. or yam are consumed by the indigenous
peoples of North-Eastern region of India as a substitute for
potato and most of the species are also used in traditional
medicine. North-East region is one of the hotspot for
Dioscorea species growing in wild habitat which has not
been characterized or identified. In the present study, eight
morphologically distinct species of Dioscorea belonging
to section Enantiophyllum, Botryosicyos and Opsophyton
were subjected to molecular characterization and
phylogenetic assessment using three marker genes (18S
rDNA, matK and rbcL). The results of sequence
characterization of the these genes revealed that 18S rRNA
gene was highly conserved than matK and rbcL gene
sequences and hence 18Sr RNA gene can be used as better
candidates for species delimitation. The phylogenetic
analysis of the combined molecular gene sequences also
showed that the species belonging to section
Enantiophyllum were monophyletic in origin.
 

Keywords: Molecular characterization, 18S rDNA, matK, rbcL, maximum parsimony, Bayesian inference

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Year: 2019
Volume: 79
Issue: 1
Article DOI: https://doi.org/10.31742/IJGPB.79.1.11
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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