Indian Society of Genetics & Plant Breeding

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Micronuclei assay as biomarker for ethyl methane sulphonate (EMS) genotoxicity in Eclipta alba (L.) Hassk.

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The eukaryotic cells are complemented with a single
nucleus. However, several cells show abnormal small nuclei
known as micronuclei, with addition to the main nucleus.
These anomalous small nuclei along with main nucleus
which may be the result of exposure of cell to cytotoxic
agents (Chemicals/physical mutagens). These mutagens
also cause structural or numerical chromosomal aberrations.
The treatment of EMS, a chemical mutagen, induces
micronuclei formation and nuclear as well as chromosomal
peculiarities in
Eclipta alba. The effect of micronuclei is
discussed in terms of cytotoxicity of genotoxins and several
evidences of nuclear polymorphism illustrated due to the
mutagenic effect of EMS. Pollen viability was also examined
through pollen fertility. Micronuclei causes the genomic
instability and elimination of genome which is effectively
put to use in breeding programmes for the creation of
aneuploids and also for haploid lines.
  

Keywords: MN (Micronuclei), EMS (Ethyl methane sulphonate), instability, pollen fertility, genotoxins

Info

Year: 2020
Volume: 80
Issue: 3
Article DOI: 10.31742/IJGPB.80.3.11
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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