Indian Society of Genetics & Plant Breeding

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Large-scale production, field performance and RAPD analysis of micropropagated sugarcane plants

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An improved procedure has been developed for the
micropropagation of true-to-type plants of two early
maturing varieties of sugarcane, CoH92 and CoH99. The
protocol Involved (i) growth and proliferation of shoot tip
explants in MS medium containing gibberellic acid,
indole-3-acetic acid and kinetin, (ii) 3-6 rounds of shoot
multiplication in MS medium enriched with 6-
benzylaminopurine and kinetin, (iii) rooting In MS medium
with (a-naphthalene acetic acid and sugar at higher
concentrations, and (vi) hardening of plantlets and their
transplantation into 1:1 mixture of unsterilized sand and
soil under natural conditions. Shoot multiplication and
rooting media contained food grade cane sugar and
Isubgol™ as cheaper substitutes in place of sucrose
(pure grade) and agar, respectively. This procedure does
not require expensive equipment and facilities such as
water purification units, greenhouse, polyhouse, etc. Plants
propagated through micropropagation and conventional
means using setts compared well for various agronomic
(cane length, cane weight, number of internodes per cane,
internode length) as well as sugar yield/quality traits
(purity and CCS). Micropropagated plants had relatively
higher number of millable canes, but they were thinner
than the conventionally propagated cane. Plants
propagated through setts of the micropropagated plants
were genetically stable for all the traits. RAPD marker
analysis using 20 primers clearly established the clonal
fidelity in >90% of micropropagated plants.
 

Keywords: Sugarcane, micropropagation, field performance, clonal fidelity, RAPD analysis.

Info

Year: 2004
Volume: 64
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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