Indian Society of Genetics & Plant Breeding

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ISOZYME CHANGES DURING MORPHOGENESIS IN THE IN VITRO CULTURES OF PANICUM MAXIMUM J.

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Callus cultures derived from mature embryos and inflorescence explants of guinea
grass [cv. GG2), Panicum maximum J. at different stages of development were used
to study the patterns of iso esterases and iso acidphosphatse, Isozyme patterns were
found to vary with all developmental stages studied. Twelve isoesterases were
detected in the embryogenic calli. With the germination of embryoids into plantlets,
only two of these isozymes were retained. Acid phosphatase was found to be organ
and tissue specific. The novel isozymes that appeared in the embryogenic and
organogenic calli can be used as marker to distinguish between embryogenic and
non-embryogenic calli.
 

Info

Year: 1999
Volume: 59
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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