Indian Society of Genetics & Plant Breeding

Important

INDUCED VARIABILITY IN HOMOZYGOUS AND HETEROZYGOUS GENOTYPES OF TOBACCO

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Dry seeds of homozygous pure breeding lines of chewing tobacco (Nicotiana tabacum
L.), Sona, bandi and their Fl hybrid were treated with chemical mutagen EMS at
three concentrations (0.1, 0.3 and 0.5%) for 12 h. In Ml generation, based on seed
germination and seedling growth, homozygous material was found to be more
sensitive than heterozygous material. Complete seed mortality was observed following
treatment with 0.5% EMS. In M2 generation, range widened for all characters in
different populations. Mean values increased in F2 over midparent but was
significantly lowered in M2 and F2 M2 for all the characters except total cured leaf
yield. There was no significant difference in mean leaf thickness which marginally
decreased in F2 and increased in M2 and F2 M2. Significant increase in nicotine
content was observed in FI and M2 but in FI M2 it decreased significantly. A
significant increase in variation was evident in F2 and MI for all characters but in
FI MI it was so for leaf breadth and first grade leaf yield. The magnitude of the
induced variation was in order of MI < FI < FI MI. Variations from hybridization
and mutation were generally not cumulative.
 

Keywords: Tobacco, Nicotiana tabacum, mutation, induced variability.

Info

Year: 1998
Volume: 58
Issue: 1
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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