Indian Society of Genetics & Plant Breeding

Important

INDUCED MUTATIONS IN CHICKPEA (CICER ARIETINUM L.) I. COMPARATIVE MUTAGENIC EFFECTIVENESS AND EFFICIENCY OF PHYSICAL & CHEMICAL MUTAGENS

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Mutagenic effectiveness usually means the rate of mutation as related to dose.
Mutagenic efficiency refers to the mutation rate in relation to damage. Studies on
comparative mutagenic effectiveness and efficiency of two physical (gamma rays
and fast neutrons) and two chemical mutagens (NMU and EMS) on two desi (G
130 & H 214), one kabuli (C 104) and one green seeded (L 345) chickpea (Cicer
arietinum L.) have been reported. The treatments included three doses each of gamma
rays (400, 500 and 600 Gy) and fast neutrons (5, 10 and 15 Gy) and two concentrations
with two different durations of two chemical mutagens, NMU (0.01% 20h and 0.02%
8h) and EMS (0.1% 20h and 0.2% 8h). Results indicated that chemical mutagens,
particularly NMU are not only more effective but also efficient than physical
mutagens in inducing mutations in chickpea. Mutagenic effectiveness and efficiency
showed differential behaviour depending upon mutagen and varietal type. Chemical
mutagens were more efficient than physical in inducing cholorophyll as well as
viable and total number of mutations. Among the mutagens NMU was the most
potent, while in the physical, gamma rays were more effective. Out of four mutagens,
NMU was the most effective and efficient in inducing a high frequency and wide
spectrum of chlorophyll mutations in the M2 followed by fast neutrons. While
gamma rays showed least effectiveness, EMS was least efficient mutagens. Major
differences in the mutagenic response of the four cultivars were observed. The
varieties of desi type were more resistant towards mutagenic treatment than kabuli
and green seeded type.
 

Keywords: Chickpea, mutagenic effectiveness and efficiency, chlorophyll mutations, frequency and spectrum

Info

Year: 1998
Volume: 58
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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