Indian Society of Genetics & Plant Breeding

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IDENTIFICATION OF SESAMUM ALATUM X SESAMUM INDICUM HYBRID USING PROTEIN, ISOZYME AND RAPD MARKERS

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With the objective of transferring phyllody disease resistance, hybridization has
been done between Sesamum alatum and S. indicum. Identical chromosome number
of the species, ambiguous morphological characters and lack of segregation in F2
necessitated to look for molecular markers to establish the hybridity. SDS-PAGE
of seed protein revealed transfer of five male-specitic proteins to the putative hybrid.
Further, inheritance of the est D locus of esterase, prx A and prx E loci of peroxidase
and RAPDs of decamer random primers have not only establishing the hybridity
but were also found to be useful as markers to distinguish the hybrids from the
seIfed progenies.
 

Keywords: Sesamum a/alum, Sesamum indicum, molecular markers

Info

Year: 1997
Volume: 57
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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