Indian Society of Genetics & Plant Breeding

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Genetics of white rust resistance in Indian mustard (Brassica juncea L.) and its validation using molecular markers

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White rust resistance loci (AcB1-A4.1 and AcB1-A5.1)
associated with intron polymorphic (IP) markers i.e.
At5g41560 and At2g36360, respectively, were used for
validation of P
1, P2, F1, F2, BC1F1 and BC2F1 generations.
The donor parents namely, Bio-YSR and BEC-144 produced
desired banding pattern of 430 and 750 bp while recipients
viz., NRCHB 101 and DRMR-150-35 exhibited different
pattern from donors confirming white rust resistance loci
4.1 and 5.1 with marker At5g41560 and At2g36360,
respectively. Confirmation of these set of two IP markers in
the parents and F
1s lead us to further screening of selected
F
2, BC1F1 and BC2F1 populations. Available data on white
rust reaction in different generations under study revealed
that single dominant gene is responsible for white rust
resistance. Potential of molecular markers in developing
white rust resistant genotypes is proved under present
study.
  

Keywords: Marker assisted selection, Brassica juncea, white rust resistance, marker validation

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Year: 2020
Volume: 80
Issue: 3
Article DOI: 10.31742/IJGPB.80.3.6
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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