Indian Society of Genetics & Plant Breeding

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Genetic studies on seed coat permeability and viability in RILs derived from an inter-specific cross of soybean [Glycine max (L.) Merrill]

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Soybean seeds loss viability very rapidly during ambient
storage in the tropical and sub-tropical environments. In
this study, interrelationship between seed coat permeability
and viability over periods of ambient storage was assessed
using a set of 217 recombinant inbred lines (RIL) developed
from an inter-specific cross between wild type (Glycine
soja) accession DC2008-1 and cultivated (G. max) variety
DS9712. G. soja seeds were tiny, black, impermeable and
highly viable while G. max seeds were large, yellow,
permeable and poorly viable during ambient storage. Seed
coat permeability and viability of the fresh, one-year and
two-year-stored seeds (stored in room temperature, av.
25±2°C and 65±5% RH) were tested as per standard protocols
in completely randomized design with two replications.
Significant variation was found among genotypes for the
seed viability, permeability, periods of storage and their
interactions. Permeability of the seed coat increased with
the period of storage. In the fresh, one-year and two-yearstored seeds, the seed coat permeability was 62.87, 75.17
and 90.52%, respectively. Viability of the seeds was
negatively correlated with period of storage and seed size.
In the fresh, one-year and two-year-stored seeds, average
viability was 90.7, 75.6 and 54.1%, respectively. Scanning
electron microscopy (SEM) indicated presence of intact
hilum, strong hourglass cells and non-cracked seed coat
in the highly viable seeds. A set of 24 RILs were found that
maintained higher viability (>80%) with varying degree of
permeability after two years of storage. Among the highly
viable RILs, more were black seeded. RIL Nos. 7-12-3, 7-24-
1, 13-2-2, 13-31-4 found to maintain both viability and
permeability in higher order during storage and would pave
the way for development of soybean genotypes with high
viability and permeability.
 

Keywords: Seed coat ultrastructure, imbibition, hourglass cells, germination, RILs, scanning electron microscopy

Info

Year: 2019
Volume: 79
Issue: 1
Article DOI: https://doi.org/10.31742/IJGPB.79.1.7
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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