Indian Society of Genetics & Plant Breeding

Genetic diversity analysis of exotic and Indian accessions of common bean (Phaseolus vulgaris L.) using RAPD markers

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Genetic diversity of forty-six common bean (Phaseolus
vulgaris
L.) accessions of diverse geographical origin was
studied using RAPD markers. Four out of the twenty
primers screened showed polymorphism across present
set of genotypes. A total of 43 DNA amplicons were
scored using these 4 primers. Seventy-seven per cent of
the amplification products showed polymorphism,
indicating fair amount of variation at the DNA level among
these accessions. The genotypes shared 59% genetic
similarity among themselves. Cluster analysis delineated
the genotypes into four groups. Twenty accessions from
United States clustered in two separate groups, which
possibly correspond to two well established phaseolus
gene pools. Some of the accessions showed promise
against the widely prevalent races of
Colletotrichum
Iindemuthianum.
Morphological variation for seed colour,
shape and size showed no correspondence with molecular
diversity.

Keywords: Common bean, genetic diversity, RAPD markers, anthracnose

Info

Year: 2006
Volume: 66
Issue: 4
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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