Downy mildew susceptible line Tifton 23A and Tifton 23B alongwith ten other
resistant near isogenic derivatives of Tift 23A showed highly polymorphic esterase
isozyme banding pattern by isoelectric focussing (pH 3.5-10.0) at three stages of
growth. AT D2 all resistant near isogenic lines in both the sterile and fertile
backgrounds were distinctly discriminated by a band with high intensity at pI 6.1
and low intensity at pI 6.9. All resistant lines except Lll1A and Lll1B were also
characterized by intense band at pI 7.3 though band intensity in 126D2A and 126D2B
was quite low. Discrimination of A and B lines of every pair was easily possible
at D1 and D3 stages. The effect of sterile and fertile cytoplasms and nucleocytoplasmic
interaction in estarase isozyme expression alongwith the role of isozyme in growth
and development has been discussed.
Keywords: Pearl millet, downy mildew, esterase isozyme, cytoplasmic male sterility, disease resistance.
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
S. R. THAKUR AND B. R. MURTY info_circle