DNA fingerprinting of Mysore Local and V-1 cultivars of mulberry (Marus spp.) with RAPD markers

Published on Aug 04, 2018 02:26 AM

DNA fingerprinting was successfully applied to distinguish
two popular mulberry (Morus spp.) cultivars, viz., Mysore
Local and V-1. RAPD analysis of 12 collections of each
of these two cultivars derived from clones of different
sources using 12 oligonucleotide random primers
generated 73 applicons of which 40 were monomorphic
and the rest 33 were polymorphic (45%). All the primers
produced typical banding profiles for each of the cultivar
suggesting the usefulness of the technique in DNA
fingerprinting and cultivar identification. The genetic
distance between these two cultivars based on the RAPD
data set was estimated as 0.292, which is low in comparison
to the morpho-agronomical difference, suggesting a narrow
genetic base of the crop.

Keywords: Mulberry, DNA fingerprinting, RAPD marker, cultivar identification

Info

Year: 2002
Volume: 62
Issue: 3
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

V. Girish Naik, A. Sarkar and N. Sathyanarayana

Download PDF