In an Induc;ed mutation study In blackgnm digitonin wu applied u a pretreatment
chemical just before the treatment with the mutagen. ethyl methanesulphonate (EMS)
to enhance the mutagenic effect of EMS through greater uptake of the mutagen
Into the cells. The M2 populations were screened for macro- and micro- mutations.
The frequency and spectrum of total macro-mutations were substantially Increased
by digitonin-EMS treatment compared to EMS treatment alone. There was also
Increue in micro- mutations by digitonin pretreatment for almost all the quantitative
characters except seeds per pod and single-plant yield.
Keywords: Vigna mungo, blackgram, Digitonin, EMS, enhancer, mutagenicity.
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906
B. R. SINGH AND B. K. MOHAPATRA info_circle