Indian Society of Genetics & Plant Breeding

Important

Differential expression profiling of defense related genes for Leaf Curl Virus (ChiLCV) in resistant and susceptible genotypes of Chiili

Published on

The present investigation was undertaken to study the
expression of eight defense related genes in leaf curl
resistant line DLS-Sel-10 and susceptible line Phule Mukta
after different days post inoculation (dpi) with viruliferous
white flies to understand their role in resistance to chilli
leaf curl virus (ChiLCV). The expression level of Ca PPO, Ca
AsPer, Ca ATP/ADP and CaTopoII was observed to be higher
in resistant genotype DLS-Sel-10 than the susceptible Phule
Mukta at all the time points studied. Expression of CaNBSLRR increased up to 12 dpi while that of Ca Thionin, and Ca
SKP1 increased up to 24 dpi in the resistant line, thereafter
it started declining. The CaSPI expression did not show
any specific pattern in both the test plants. The heat map
clustered all the genes under study into two major clusters
based on their expression profiles, one comprising
CaAsPer, Ca-Thionin, CaATP/ADP transporter, CaPPO and
CaTopoII while other group comprised CaSKPI, Ca NBS
and CaSPI. The challenge inoculation of the test genotypes
also revealed that viral titre increased at a much slower rate
in DLS-Sel-10 than Phule Mukta, suggesting thereby that
DLS-Sel-10 is resisting the accumulation of ChiLCV and
has a more active defense machinery than Phule Mukta.
  

Keywords: Chilli, ChiLCV, defence genes, expression profiling, qPCR

Info

Year: 2020
Volume: 80
Issue: 3
Article DOI: 10.31742/IJGPB.80.3.10
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle

Download PDF