Indian Society of Genetics & Plant Breeding

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Development and characterization of STMS molecular markers and genetic diversity analysis of mungbean [Vigna radiata (L.) Wilczek]

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Mung bean is an important crop in Asia because of its high
protein content and other economic uses. However,
because of the unavailability of polymorphic DNA markers,
genomic research of mung bean is lacking. In this study,
we developed and characterised simple sequence repeat
(SSR) molecular markers by screening SSR-enriched partial
genomic libraries with SSR probes and used them to analyse
the genetic diversity of mung bean. Thus, we isolated,
cloned, sequenced a genomic library that contained
microsatellite loci from the mung bean variety ‘MCV-1’. The
polymorphisms of microsatellite loci were evaluated using
the unweighted pair group method of arithmetic means,
and MDS cluster analysis showed genetic relationships in
a panel of 96 mung bean core collection genotypes. Genetic
diversity analysis results showed contrasted levels of
variability within cultivated and wild accessions. A total of
98 alleles were detected using 19 polymorphic markers,
with an average of 4.9 alleles per locus, whereas observed
heterozygosity ranged from 0.1 to 0.5, with a mean of 0.42
per locus. The number of alleles and the high level of
polymorphism make these new markers useful for gene
tagging, diversity analyses and marker assisted selection
in mung bean.
 

Keywords: Microsatellite marker, molecular characterisation, polymorphism, genetic variation

Info

Year: 2019
Volume: 79
Issue: 1
Article DOI: doi.org/10.31742/IJGPB.79.1.9
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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