Indian Society of Genetics & Plant Breeding


Developing rice hybrids for temperate conditions using three line approach

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Exploitation of heterosis in the form of hybrid rice is yet to
be exploited at commercial level in the temperate regions
of India. The present study is attempted to develop rice
hybrids for the temperate conditions of Kashmir valley by
utilizing the cytoplasmic genetic male sterility system
(CGMS). Among the four CMS lines evaluated, the genotype
SKUA-7A and SKUA-11A had desirable performances. The
24 test crosses developed involving these CMS lines were
evaluated for spikelet fertility and pollen fertility. Moreover,
the highest standard heterosis for grain yield and other
yield attributes over the check varieties (Jehlum and
Shalimar Rice-3) was recorded in the cross combination
K15-34 followed by K15-31 and K15-40. Pollen parents of all
the three test crosses on screening with RM-6100, DDRMRf3-10 and DRCG-Rf4-14 markers revealed presence of both
Rf3 and Rf4 genes responsible for fertility restoration.
Besides, 39 genotypes were screened for fertility restoration
and the lines RL-2, RL-5, RL-6, RL-7, RL-10, Pusa Sugandh-
3 and Kohsar were identified to carry homozygous alleles
at both Rf3 and Rf4 loci. These genotypes can thus be
used as promising restores for the development of
temperate rice hybrids.

Keywords: Hybrid rice, cytoplasmic male sterile lines, restorer lines, Rf3, Rf4


Year: 2019
Volume: 79
Issue: 1
Article DOI:
Print ISSN: 0019-5200
Online ISSN: 0975-6906



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