Indian Society of Genetics & Plant Breeding

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Detection and characterization of polymorphic simple sequence repeats markers for the analysis of genetic diversity in Indian mungbean [Vigna radiata (L.) Wilczek]

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Mungbean is a widely cultivated grain legume in Asia, Africa
and South America. In this study, 52 varieties of mungbean
were profiled with 39 polymorphic SSR primers after
screening a total 315 SSR primers. A total of 96 alleles were
scored from the 39 primers with an average of 2.46 alleles
per locus indicating the low diversity among varieties. The
Nei’s genetic diversity index and the Shannon information
index of SSR primers varied from 0 to 0.649 and 0 to 1.169,
respectively. The results showed that the potential
transferability of adzuki bean primers (83.3%) was greater
than the cowpea primers (25%). The varieties profiled were
grouped into four major clusters. But the clustering pattern
did not reflect on their geographical origin. Further, the
AMOVA indicated presence of moderate genetic
differentiation among groups compared to higher
differentiation among varieties within populations. The SSR
markers identified here will add valuable genomic resources
for germplasm characterization, cultivar identification and
assessment of genetic diversity of mungbean varieties.
 

Keywords: AMOVA, genetic diversity, mungbean, marker transferability, SSR markers

Info

Year: 2018
Volume: 78
Issue: 1
Article DOI: 10.5958/0975-6906.2018.00013.5
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Ashok Chaudhury info_circle
Suraj Umdale info_circle
Ratna Kumar info_circle
Bhavana Tiwari info_circle
Sanjay Kumar info_circle
Ambika B. Gaikwad info_circle
K. V. Bhat info_circle

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