Indian Society of Genetics & Plant Breeding

Important

Demonstration of CRISPR-cas9-mediated pds gene editing in a tomato hybrid parental line

Published on

CRISPR-cas9 mediated gene editing is a powerful tool
proven for crop improvement and has great potential for
incorporating novel traits into important genetic resources.
In this study, pds gene editing in a parent line of a tomato
hybrid by using CRISPR-cas9 system was successfully
conducted. Three small guide RNAs (sgRNAs) were
designed to target three exons of a tomato pds gene and
were transformed along with a cas9 gene into the line of
interest. Observation of chimeric albino phenotypes in the
regenerated tomato shoots indicated the disruption of pds
gene. Furthermore, expression of cas9 gene was determined
by demonstration of its transcript accumulation in the
primary tomato transformants. Sequence analysis of
targeted pds exons showed point as well as deletion
mutations in the sgRNA target sites. The result confirmed
functional nature of the CRISPR-cas9 assembly used in the
present study, which will enable incorporation of novel
traits directly into the parents of tomato hybrids.
 

Keywords: Tomato, pds, CRISPR-cas9, sgRNA, gene editing

Info

Year: 2018
Volume: 78
Issue: 1
Article DOI: 10.5958/0975-6906.2018.00016.0
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

info_circle
Anjanabha Bhattacharya info_circle
Shalu Choudhary info_circle
Rahul Pathak info_circle
Vrushali Gawade info_circle
Bhavesh Palan info_circle
Leela Alamalakala info_circle
Venugopal Mikkilineni info_circle
Bharat Char info_circle

Download PDF