Indian Society of Genetics & Plant Breeding


Cloning and characterization of nutrient deficiency and salinity stress responsive TaCBL4 gene from bread wheat (Triticum aestivum L.)

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Environmental stresses, such as drought, salinity, extreme
temperatures and nutrient deficiencies affect plant
productivity adversely. Calcineurin B-like proteins (CBLs)
and CBL-interacting protein kinases (CIPKs) are
components of calcium mediated stress response pathways
in many plants. In the present study, TaCBL4 gene
homologous to salt stress responsive Arabidopsis CBL4
(AtSOS3) was cloned from wheat and sequence
characterized. The predicted protein of TaCBL4 showed
95, 83, 70 and 61% similarity with amino acid sequences of
barley, rice, maize and Arabidopsis CBL4, respectively,
demonstrating that CBL4 genes are conserved between
dicots and monocots. TaCBL4 protein consists of three
EF-hand calcium binding motifs, a characteristic feature of
calcium sensor proteins. The modelled image of TaCBL4
protein was highly identical to the already resolved structure
of the AtCBL4 protein. In addition to salinity stress, the
expression of TaCBL4 gene was also up-regulated by
deficiency of major nutrients, namely, nitrogen, phosphorus
and potassium. This suggests that TaCBL4 may serve as
node for signaling crosstalk between salinity and nutrient
deficiency stress signaling pathways and nutrient
homeostasis under salinity stress. The present study lays
foundation for functional studies on role of TaCBL4 gene
in nutrient use efficiency and for its further utilization in
breeding new wheat varieties with enhanced nutrient use
efficiency and salinity tolerance.

Keywords: Wheat, CBL, SOS, salinity, nutrient stress


Year: 2016
Volume: 76
Issue: 2
Article DOI: 10.5958/0975-6906.2016.00029.8
Print ISSN: 0019-5200
Online ISSN: 0975-6906


S. K. Jha info_circle
V. Chinnusamy info_circle
R. K. Sairam info_circle

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