Indian Society of Genetics & Plant Breeding

Assessment of genetic diversity among pigeonpea genotypes using SSR markers

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Twenty two SSR markers of different crop species origin
were used to assess polymorphism through their SSR
fingerprinting of 16 cultivated pigeonpea genotypes. Four
hundred twenty five bands were amplified in all the sixteen
genotypes. A total of 46 SSR fragments were amplified.
Eight primers showed 100% polymorphism. Based on
dendrogram constructed using the similarity coefficient
values, 16 genotypes were grouped into two distinct
clusters. Cluster I comprises mostly late duration
genotypes while cluster II comprises medium duration
genotypes except CO-6 and Bahar. Both the clusters and
sub-cluster in the dendrogram were supported by high
bootstrap values, thus indicating that the SSR could be a
good choice to classify the genotypes. Genotypes with
high molecular diversity could be used in breeding
methodologies and development of gene pools with broad
genetic base. The genotype specific bands developed by
the SSR primers could also be used for cultivar
identification.
 

Keywords: Cajanus cajan, DNA isolation, fingerprinting, genetic diversity, SSR markers

Info

Year: 2008
Volume: 68
Issue: 3
Article DOI: N/A
Print ISSN: 0019-5200
Online ISSN: 0975-6906

Authors

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Rama Kant info_circle
Sahil Mehfooz and S. Dutta info_circle

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