Indian Society of Genetics & Plant Breeding

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An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

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In this study, the Agrobacterium infection medium, infection
duration, detergent, and cell density were optimized. The
sorghum-based infection medium (SbIM), 10-20 min
infection time, addition of 0.01% Silwet L-77, and
Agrobacterium optical density at 600 nm (OD600), improved
the competence of onion epidermal cells to support
Agrobacterium infection at >90% efficiency. Cyclindependent kinase D-2 (CDKD-2) and cytochrome c-type
biogenesis protein (CYCH), protein-protein interactions
were localized. The optimized procedure is a quick and
efficient system for examining protein subcellular
localization and protein-protein interaction.
  

Keywords: Agrobacterium-mediated method, Allium cepa (onion), BiFC, Transient gene function assay

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Year: 2020
Volume: 80
Issue: 3
Article DOI: 10.31742/IJGPB.80.3.17
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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