Indian Society of Genetics & Plant Breeding

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Amplified fragment length polymorphism (AFLP) based diversity in advanced breeding lines of cotton (Gossypium hirsutum L.)

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Amplified fragment length polymorphism (AFLP) analysis
was carried out in 29 advanced breeding lines of cotton
(G. hirsutum L.) for diversity analysis. Total genomic DNA
isolated following CTAB method was purified and
subsequently used for AFLP analysis employing
fluorescent dye labeling and detection technology. Using
308 amplicons generated by three AFLP primer
combinations, Jaccard's similarity estimates between all
possible pairs of 29 lines were calculated. High level of
diversity was observed in the studied material. Cultivars
Pusa 8-6 and RS 810 had the maximum dissimilarity
between them, whereas lines RAH 30 and NOH 1001 were
naving the highest similarity. Cluster analysis revealed
lines AKH 081, NOH 1010, RS 810, RST 13 and Pusa 8-6
to be the most distinct ones. Advanced breeding lines
from different sources were found to be interspersed and
no source-wise clustering was evident.
 

Keywords: Cotton, amplified fragment length polymorphism (AFLP), genetic diversity

Info

Year: 2004
Volume: 64
Issue: 2
Article DOI: NA
Print ISSN: 0019-5200
Online ISSN: 0975-6906

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